Sandwich probes: two simultaneous reactions for templated nucleic acid detection
نویسندگان
چکیده
منابع مشابه
Sandwich probes: two simultaneous reactions for templated nucleic acid detection.
Fluorescence-quenched nucleic acid probes with reactive moieties at both the 5' and 3' ends are synthesized and tested for reaction with two adjacent nucleophile-containing DNAs. These probes improve signal to background over singly reactive probes and can discriminate single nucleotide polymorphisms in the target DNA or RNA.
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ژورنال
عنوان ژورنال: Chemical Communications
سال: 2010
ISSN: 1359-7345,1364-548X
DOI: 10.1039/c0cc01968b